Silver Diamine Fluoride와 요오드화 칼륨 도포 후 상아질 표면 거칠기와 초기 우식원성 세균막 형성
Surface Roughness of Dentin and Formation of Early Cariogenic Biofilm after Silver Diamine Fluoride and Potassium Iodide Application
김해니, 박호원, 이주현, 이시영,
소속 상세정보
김해니 ( Kim Hae-Ni ) - Gangneung-Wonju National University College of Dentistry Department of Pediatric Dentistry
박호원 ( Park Ho-Won ) - Gangneung-Wonju National University College of Dentistry Department of Pediatric Dentistry
이주현 ( Lee Ju-Hyun ) - Gangneung-Wonju National University College of Dentistry Department of Pediatric Dentistry
이시영 ( Lee Si-Young ) - Gangneung-Wonju National University College of Dentistry Department of Microbiology and Immunology
Abstract
이 연구는 우식원성 세균막 형성과 상아질 표면 거칠기에 대한 Silver diamine fluoride와 요오드화 칼륨(KI)의 영향을 실험실 환경에서 평가하는 것이다.
인공 우식을 유발한 48개의 Bovine dentin 시편을 제작하여 대조군, SDF 도포, SDF와 KI 도포(SDFKI)의 3개 군으로 나누었다. 각 군당 10개의 시편의 상면에 Streptococcus mutans , Lactobacillus casei 및 Candida albicans 를 포함하는 다종 우식원성 세균을 24시간 동안 배양 후 세균 수를 측정하였다. 나머지 시편은 원자 힘 현미경과 주사전자현미경으로 관찰하였다.
SDF와 SDFKI 도포 시 대조군에 비해 세균 수가 유의하게 감소했으며, KI는 SDF의 항균 효과를 유의하게 저해하지 않았다. 현미경 관찰 시 SDF와 SDFKI 도포 후 생성되는 입자가 상아질 표면에 침착 되는 것을 관찰했으나 표면 거칠기는 세 군 간 유의한 차이가 없었다.
This study aimed to evaluate the effect of silver diamine fluoride (SDF) and potassium iodide (KI) on the formation of cariogenic biofilm and surface roughness in vitro.
A total of 48 bovine dentin specimens with artificially induced caries were prepared and divided into 3 groups of 16: untreated control, SDF-treated, and SDF-treated followed by KI (SDFKI). Ten specimens from each group were used to observe microbial adhesion. Multispecies cariogenic biofilms including Streptococcus mutans , Lactobacillus casei , and Candida albicans were cultured on the specimens. Microbes were cultured for 24 hours, and the colony-forming unit was calculated. The remaining specimens were observed by atomic force microscope and scanning electron microscope (SEM).
The number of bacteria was significantly lower in the SDF and SDFKI groups. KI did not inhibit the antibacterial activity of SDF significantly. SEM images showed particles generated after SDF and SDFKI application were deposited on the dentin, but there was no significant difference in surface roughness between the 3 groups.
This study confirmed that SDF and SDFKI application did not have a significant effect on the surface roughness of dentin, but effectively inhibited the formation of the early cariogenic bacterial film after 24 hours compared to the control.
키워드
Silver diamine fluoride; Potassium iodide; Cariostatic agents; Biofilms; Surface roughness
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